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Look at most relevant Class vp software operation manual websites out of 3.51 Million at KeyOptimize.com. Class vp software operation manual found at files.instrument. The effects of cadmium chloride on secondary metabolite production in Vitis vinifera The compounds were quantified using Shimadzu CLASS-VP software..

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PURPOSE: To provide a procedure to operate and maintain the Shimadzu make Class VP series Gradient HPLC system. SCOPE: This procedure is applicable for the GRADIENT HPLC, Shimadzu -Class VP series used in the Quality Control department III. RESPONSIBILITY: It is the responsibility of all the personnel involved in the analysis using of the system IV.

DOCUMENT REFERENCE: SOPs: Core procedure: Calibration of Equipment Forms: Calibration of HPLC V. OPERATION: 1.0 PRELIMINARY CHECK: 1.1 Check that the instrument is clean and free from dust, if not, clean with a soft cloth duster. 2.0 THE LC-10 AVP SERIES HPLC WORK STATION CONSISTS OF: 2.1. LC-10AT VP Pump 2.2. SPD-10 AVP UV vis detector 2.3. SIL-HT- A System controller and auto sampler 2.4.

Computer with Class VP software version 6.10 3.0 BASIC OPERATION: 3.1. Switch ‘ON’ the main for LC-10 pump 3.2. Switch ‘ON’ the main for auto injector 3.3. Switch ‘ON’ the main for UV vis detector 3.4. Switch ‘ON’ the system controller and the computer.

After switching ‘ON’ all the components, finally switch ‘ON’ the computer. Default screen appears on the respective screen modules. Double click on the (Shimadzu class VP) icon in the screen. Double click on Instrument 1 icon. Here for the beep sound, which indicates the communication between the system and the software. OPERATING PROCEDURE FOR LC-10AVP PUMP 3.10.

Open the drain valve of the pump. Press the purge key. Ensure that there are no air bubbles in the flow line and press purge/pump key to stop purging. Close the drain valve of the pump. SYSTEM SOFTWARE 3.11 Check the method name displayed on the top of the screen. 3.12 Click on the method option, click the instrument set up.

Click on the pump, set the A. Flow in ml/min. P.max in kgf/cm2. 3.13 Click on the detector SPD-10 AVP, icon.

Set the wavelength in nm and run time in min. and save the method. 3.14 After setting the above parameters, click on download at the bottom of the screen. 3.15 Observe for the message displayed on the screen.

Click the OK icon and close the screen. This indicates the software successfully downloaded the set information to the system. 3.16 Click on sequence.

Click on edit, edit table will appear. Set the vial number. Repetitions from the vial (no.

Of injections) sample ID, File name and finally Description. AUTOZERO/PURGE INJECTOR / RINSE INJECTOR/INSTRUMENT ‘ON/OFF’ 3.17 Go to control option, click the direct control. Direct control message will appear on the top left corner of the screen. Click on the instrument on/off icon.

It activates the whole system ACT LED will glow on the system controller. Before injecting a sample observe for the base line by pressing preview run in control option. To start a run, press the single run into the control option or blue arrow icon on the main screen. If the injections are in sequence mode, press the double green arrow button on the main screen.

To stop the single run, press the stop run column in the control option. RESULTS & PRINTOUTS 3.21 Click on the Shimadzu class-vp button. 3.22 Double click on the instrument off line processing instrument 1 will appear. Double click instrument 1.

3.23 On double click, instrument 1 (off line). The method used and the data file namewill appear on the top of the screen. 3.24 To open, select data file, click file, under file option, click data, press open, data files will appear. Select the data and press open, chromatogram will appear on the screen. 3.25 To check the results, press view and then method custom report.

3.26 Class-vp custom report will appear on the screen. 3.27 To print click the right button of the mouse and press print. CALIBRATION Calibration frequency: Once in three months 1.0 Calibration of the pump 1.1 Check the flow rate of the filtered and degassed Purified water as follows: 1.2 Disconnect any column if connected to the system and connect restriction capillary 1.3 Set the flow of the pump at 1.0 mL per minute. Keep the system at this flow rate for about 5 minutes to equilibrate the system. 1.4 Collect the volume of water delivered for 5 minutes and determine the weight of the water. 1.5 Convert the weight in to volume by the following table: Temperature, Volume, in mL, Temperature, Volume, in mL, °C of 1g of water °C of 1g of water 20 1.0028 26 1.0042 21 1.003 27 1.0045 22 1.0032 28 1.0047 23 1.00345 29 1.005 24 1.0037 30 1.0053 25 1.0039 1.6 Repeat the above for 2 and 3 mL flow rates. 1.7 Acceptance criteria: Flow rate: + 0.1mL 2.0 Column compartment thermostat / oven calibration.

2.1 Disconnect any column if connected to the system. 2.2 Purge the system with Purified water to remove any solvents and previous buffer salts.

2.3 Connect a restricted capillary of dimension 2m x 0.12 mm ID in place of column. 2.4 Keep a flow rate of 1.0 mL/minute. 2.5 Keep the sensor wire of the traceable digital thermometer in the column thermostat/ oven.

2.6 Set the temperature at 20.0°C.Wait till the set temperature is attained and the temperature display on the instrument is stable. 2.7 Wait for 5 minutes before readings are taken so that the temperature on the Instrument and that displayed on the thermometer are stable. 2.8 Note down the temperature displayed by the instrument and the thermometer. 2.9 Repeat the steps 2.6 to 2.8 for temperature settings at 30.0°C, 40.0°C, 50.0°C,60.0°C & 70.0°C.

2.10 Acceptance Criteria: The difference between the set temperature and the displayed temperature on the thermometer should not be more than ± 2.0°C. 3.0 BASE LINE CHECK (NOISE & DRIFT) 3.1 Connect the restriction capillary and check for any leakages at all possible points. 3.2 Allow the system to stabilize for few minutes by passing purified water through the restriction capillary and the detector. 3.3 Check monitor base line before starting the Noise & drift test.Once the base line is stabilized proceed to the Noise & drift test as follows.

3.4 Click the base line button in the LC set up or Select Control Baseline Check dialog to display a base line check dialog box. 3.5 Enter the time period for start, end time and Threshold values for the Noise and Drift test Use ASTM method to evaluate the above test. 3.6 The Threshold values for Noise and drift are: 50uV for Noise 5000uV/h for Drift. 3.7 Click the start button to start the test and Click cancel to stop the test. 3.8 If the Noise & Drift values are within the specified threshold range the result will be displayed as Pass. If the measured value is larger than the threshold check the system for other maintenance in the pump and detector.

3.9 Export the result file by specifying the data path and file name and print the file from the folder. Alternatively use print screen to print the result window. Acceptance Criteria: The Noise & drift values obtained from the test should be within the set Threshold values. 4.0 DETECTOR LAMP INTENSITY CHECK: 4.1 Click on the control icon in Instrument I, then click instrument status. 4.2 Instrument status will appear on the screen.

4.3 Click on the SPD-10 AVP (Det A) icon in instrument 1. 4.4 Reference energy and sample energy will be displayed on the screen. Acceptance Criteria: Reference energy displayed should not be less than 800 mV at 220 nm. 5.0 WAVELENGTH ACCURACY: 5.1 Preparation of standard solution: Weigh accurately about 25 mg of caffeine in a 100ml volumetric flask, dissolve and dilute to volume with water. Further dilute 10ml of this solution to 100ml with water. 5.2 In the HPLC, connect the outlet of the pump into the inlet of detector.

5.3 Flush the flow cell with water. 5.4 In the ON LINE, go to instrument set up and enter the start and end wavelengths for spectrum scan. 5.5 Go to ‘Control’’ press on ‘Spectrum scan’ spectrum scan window appears. 5.6 Press background and acquire a background and spectrum. 5.7 Fill the flow cell with Caffeine solution and press ‘spectrum’ to acquire a spectrum. 5.8 Right click on the spectrum and select the mode to calculate? Max where the maximum absorbance value is observed.

5.9 Again right click on spectrum move the cursor to ‘Utility’ and save the spectrum. 5.10 Go to method, custom report, delete all the graphs, right click, insert graph, spectrum control. The template appears and another window of spectrum control appears. In that source select ‘file’ and open the spectrum data and say apply, click ‘OK’.

Right click on the graph, select axes set up and set the ‘x’ axis scale to 200 to 300 nm 5.11 Click on ‘report’ ‘print’ and ‘method custom report’. 5.12 Right click on the graph, select axes set up and set the ‘x’ axis scale to 200 to 300 nm and then take printout as explained in 8.4.10. 5.13 In the expanded spectrum printout, draw perpendicular from the maximum absorbance to the ‘x’ axis. Note down the intercept of the perpendicular on the ‘x’ axis.

Hello everybody, I know this is a question about a very old system and there have been some threads about related topics - but I did not get any useful answer there. Anyway: we have a second-hand HPLC system running with SPD-M10Avp and class-VP software (4.x-version, on windows 95). The PC looks very old and I tried to move the system to a) a windows-XP and b) a windows-98SE computer. In both cases, everything is running (I can operate the pumps for example) but I cannot get the detector connected.

My question: do I need a separate driver for the SPD-M10AVP??? (I suppose that yes - I get a warning during the WIN-boot-sequence about an unknown device etc.) If yes - where can I get one? I searched some hours already but cannot find it anywhere.

(and wonder why Shimadzu does not put such essential things on their webpage.) It would be great if somebody could give me a hint - many thanks!! Trying to recollect as worked more than 10 years back.

4.X may work with 98SE but will not with XP. Check following: a) You should have a ISA slot in the 98SE CPU.

Install the VP Interface ISA card in CPU to establish communication between SCL-10A. This driver should be available in vp software. You should also set correct IRQ, I/O settings and ensure no conflicts.

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B) You can establish communcation with SCL-10A in instrument one after connecting RS-232. 3) Install PCI-SCSI in slot and driver. 4) In instrument one Add Channel 2 PDA & select M10Avp to establish communication with PDA.

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Or an easier way out: Catch a shimadzu person get a copy of M10A software this will only control PDA & no other module. Install in 98SE. Get PCI scsi with driver.

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Establish communication and control pump etc. Best wishes, Sivaraman.